Photosynthetica 2018, 56(1):178-184 | DOI: 10.1007/s11099-017-0749-5

PsbP-induced protein conformational changes around Cl- ions in the water oxidizing center of photosystem II

J. Kondo1, T. Noguchi1,*
1 Division of Material Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan

PsbP is an extrinsic protein of PSII having a function of Ca2+ and Cl- retention in the water-oxidizing center (WOC). In order to understand the mechanism how PsbP regulates the Cl- binding in WOC, we examined the effect of PsbP depletion on the protein structures around the Cl- sites using Fourier transform infrared (FTIR) spectroscopy. Light-induced FTIR difference spectra upon the S1→S2 transition were obtained using Cl--bound and NO3--substituted PSII membranes in the presence and absence of PsbP. A clear difference in the amide I band changes by PsbP depletion was observed between Cl--bound and NO3--substituted PSII samples, indicating that PsbP binding perturbed the protein conformations around the Cl-ion(s) in WOC. It is suggested that PsbP stabilizes the Cl- binding by regulating the dissociation constant of Cl- and/or an energy barrier of Cl- dissociation through protein conformational changes around the Cl- ion(s).

Additional key words: Mn4CaO5 cluster; oxygen evolution; photosynthesis

Received: April 20, 2017; Accepted: May 31, 2017; Published: March 1, 2018  Show citation

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Kondo, J., & Noguchi, T. (2018). PsbP-induced protein conformational changes around Cl- ions in the water oxidizing center of photosystem II. Photosynthetica, 56(SPECIAL ISSUE), 178-184. doi: 10.1007/s11099-017-0749-5
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