Photosynthetica 2018, 56(1):275-278 | DOI: 10.1007/s11099-017-0760-x

The deep red state of photosystem II in Cyanidioschyzon merolae

J. Langley1, J. Morton1, R. Purchase1, L. Tian2, L. Shen2, G. Han2, J. R. Shen2,3, E. Krausz1,*
1 Research School of Chemistry, Australian National University, Canberra, Australia
2 Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences, Beijing, China
3 Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan

We identified and characterised the deep red state (DRS), an optically-absorbing charge transfer state of PSII, which lies at lower energy than P680, in the red algae Cyanidioschyzon merolae by means of low temperature absorption and magnetic circular dichroism spectroscopies. The photoactive DRS has been previously studied in PSII of the higher plant Spinacia oleracea, and in the cyanobacterium Thermosynechococcus vulcanus. We found the DRS in PSII of C. merolae has similar spectral properties. Treatment of PSII with dithionite leads to reduction of cytochrome (cyt) b559 and the PsbV-based cyt c550 as well as the disassembly of the oxygen-evolving complex. Whereas the overall visible absorption spectrum of PSII was little affected, the DRS absorption in the reduced sample was no longer seen. This bleaching of the DRS is discussed in terms of a corresponding lack of a DRS feature in D1D2/cyt b559 reaction centre preparations of PSII.

Additional key words: optical spectra; photosynthesis

Received: June 14, 2017; Accepted: August 21, 2017; Published: March 1, 2018  Show citation

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Langley, J., Morton, J., Purchase, R., Tian, L., Shen, L., Han, G., Shen, J.R., & Krausz, E. (2018). The deep red state of photosystem II in Cyanidioschyzon merolae. Photosynthetica, 56(SPECIAL ISSUE), 275-278. doi: 10.1007/s11099-017-0760-x
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