Photosynthetica 2018, 56(4):1447-1452 | DOI: 10.1007/s11099-018-0848-y

A method of a bicolor fast-Fourier pulse-amplitude modulation chlorophyll fluorometry

V. Lysenko1,*, D. Lazár2, T. Varduny1
1 Botanical Garden, Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia
2 Department of Biophysics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic

A simple method of a bicolor (multicolor), fast-Fourier, PAM chlorophyll fluorometry has been developed to obtain fluorescence induction curves. Quantum yields of PSII photochemistry were determined with blue and red simultaneously applied pulsed measuring lights for three subsequent 20-min periods of dark-, light-adaptation under actinic light and dark recovery. Measuring lights were cross-combined with blue and red actinic lights and saturation pulses. Coefficients of chromatic divergence were calculated as a ratio of the quantum yields obtained by red measuring light to that obtained by blue measuring light. Adaptation of Ficus benjamina and Hordeum vulgare leaves under blue (but not red) actinic light resulted in the sufficient increase of chromatic divergence. In addition, fraction of active, non(photo)inhibited, PSII centers was shown to be dependent on the color of measuring light. Thus, color of the light sources should be considered when reporting results of parameters evaluated from fluorescence induction curves.

Additional key words: chromatic adaptation; greenhouse lighting; dual-wavelength PAM; multi-color PAM

Received: October 30, 2017; Accepted: February 26, 2018; Prepublished online: December 1, 2018; Published: November 1, 2018  Show citation

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Lysenko, V., Lazár, D., & Varduny, T. (2018). A method of a bicolor fast-Fourier pulse-amplitude modulation chlorophyll fluorometry. Photosynthetica, 56(4), 1447-1452. doi: 10.1007/s11099-018-0848-y
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    References

    1. Baránková B., Lazár D., Nauą J.: Analysis of the effect of chloroplast arrangement on optical properties of green tobacco leaves.-Remote Sens. Environ. 174: 181-196, 2016. Go to original source...
    2. Figueroa F.L., Jerez C.G., Korbee N.: Use of in vivo chlorophyll fluorescence to estimate photosynthetic activity and biomass productivity in microalgae grown in different culture systems.-Lat. Am. J. Aquat. Res. 41: 801-819, 2013. Go to original source...
    3. Genty B., Briantais J.-M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    4. Guo Z, Wang F, Xiang X. et al.: Systemic induction of photosynthesis via illumination of the shoot apex is mediated sequentially by Phytochrome B, auxin and hydrogen peroxide in tomato.-Plant Physiol. 172: 1259-1272, 2016. Go to original source...
    5. Hogewoning S.W., Trouwborst G., Maljaars H. et al.: Blue light dose-responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light.-J. Exp. Bot. 61: 3107-3117, 2010. Go to original source...
    6. Hogewoning S.W., Wientjes E., Douwstra P. et al.: Photosynthetic quantum yield dynamics: from photosystems to leaves.-Plant Cell 24: 1921-1935, 2012. Go to original source...
    7. Jakob T., Schreiber U., Kirchesch V. et al.: Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwavelength-excitation PAM chlorophyll fluorometry: performance and methodological limits.-Photosynth. Res. 83: 343-361, 2005. Go to original source...
    8. Kitajima M., Butker W.L.: Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone.-Biochim. Biophys. Acta 376: 105-115, 1975. Go to original source...
    9. Lazár D.: Parameters of photosynthetic energy partitioning.-J. Plant Physiol. 175: 131-147, 2015. Go to original source...
    10. Lysenko V., Varduny T.: Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?-Sci. Rep. 3: 3373, 2013. Go to original source...
    11. Lysenko V.S., Varduny T.V., Simonovich E.I. et al.: Far-red spectrum of second Emerson effect: a study using dualwavelength pulse amplitude modulation fluorometry.-Am. J. Biochem. Biotech. 10: 234-240, 2014. Go to original source...
    12. Muneer S., Kim E.J., Park J.S. et al.: Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.).-Int. J. Mol. Sci. 15: 4657-4670, 2014. Go to original source...
    13. Pfündel E.E.: Deriving room temperature excitation spectra for photosystem I and photosystem II fluorescence in intact leaves from the dependence of FV/FM on excitation wavelength.-Photosynth. Res. 100: 163-177, 2009. Go to original source...
    14. Pfündel E.E., Latuche G., Meister A., Cerovic Z.G.: Linking chloroplast relocation to different responses of photosynthesis to blue and red radiation in low and high light-acclimated leaves of Arabidopsis thaliana.-Photosynth. Res. 137: 105-128, 2018. Go to original source...
    15. Schreiber U., Klughammer C., Kolbowski J.: Assessment of wavelength-dependent parameters of photosynthetic electron transport with a new type of multi-color PAM chlorophyll fluorometer.-Photosynth. Res. 113: 127-144, 2012. Go to original source...
    16. Schreiber U.: Pulse-amplitude-modulation (PAM) fluorometry and saturation pulse method: An overview.-In: Papageorgiou G.C., Govindjee (ed.): Chlorophyll a Fluorescence: a Signature of Photosynthesis. Pp. 279-319. Springer, Dordrecht 2004. Go to original source...
    17. Schreiber U., Klughammer C.: Wavelength-dependent photodamage to Chlorella investigated with a new type of multicolor PAM chlorophyll fluorometer.-Photosynth. Res. 114: 165-177, 2013. Go to original source...
    18. Suggett D.J., Goyen S., Evenhuis C. et al.: Functional diversity of photobiological traits within the genus Symbiodinium appears to be governed by the interaction of cell size with cladal designation.-New Phytol. 208: 370-381, 2015. Go to original source...
    19. Szabó M., Wangpraseurt D., Tamburic B. et al.: Effective light absorption and absolute electron transport rates in the coral Pocillopora damicornis.-Plant Physiol. Bioch. 83: 159-167, 2014. Go to original source...
    20. Yu X., Liu H., Klejnot J. et al.: The cryptochrome blue light receptors.-Arabidopsis Book 8: e0135, 2010. Go to original source...

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