Photosynthetica 2018, 56(4):1453-1458 | DOI: 10.1007/s11099-018-0836-2

Effects of endogenous ascorbic acid on resistance to high-temperature stress in excised rice leaves

Q.L. Zhang1, Y.X. Wei1, C.L. Peng1,*
1 Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou, China

Ascorbic acid (Asc) is a major plant antioxidant. L-galactono-1,4-lactone dehydrogenase (GLDH) is an enzyme that catalyzes the last step of Asc biosynthesis in higher plants. Effects of endogenous Asc on resistance to high-temperature stress were studied by using GLDH-overexpressed (GO-2) and GLDH-suppressed transgenic rice (GI-2) as experimental materials. After high-temperature treatment, the maximal quantum yield of PSII was significantly lower in GI-2, and higher in GO-2 compared to wild type rice. The content of reactive oxygen species (ROS) was the highest in GI-2. The higher Asc content resulted in lower lipid peroxidation in GO-2. The contents of chlorophyll, soluble proteins, and Rubisco large and small subunit were positively correlated to the Asc content. These results show that the higher Asc content reduced the accumulation of ROS and maintained the function of rice leaves. We suggest that the higher Asc content could improve the rice resistance to high-temperature stress.

Additional key words: ascorbic acid; high-temperature stress; reactive oxygen species; rice

Received: October 5, 2017; Accepted: January 23, 2018; Prepublished online: December 1, 2018; Published: November 1, 2018  Show citation

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Zhang, Q.L., Wei, Y.X., & Peng, C.L. (2018). Effects of endogenous ascorbic acid on resistance to high-temperature stress in excised rice leaves. Photosynthetica, 56(4), 1453-1458. doi: 10.1007/s11099-018-0836-2
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