Photosynthetica 2019, 57(3):850-856 | DOI: 10.32615/ps.2019.101

Physiological and molecular responses of maize (Zea mays L.) plants to drought and rehydration

P.Yu. VORONIN, S.N. MAEVSKAYA, M.K. NIKOLAEVA
K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya 35, 127276 Moscow, Russia

Physiological and molecular responses of maize seedlings (Zea mays L. cv. Troinaya sladost) to 5-d drought and rehydration for 48 h were investigated. Plant water status was determined by a new method of water potential measurement in mesophyll cells' apoplast in substomatal cavity (ψwa). Drought caused the changes in water status, plant growth, the rates of photosynthetic CO2/H2O gas exchange, and metabolism of carbohydrates and proline. The increase in carbohydrate and proline content under drought was observed simultaneously with the decline in ψwa. Rewatering of seedlings for 24 and 48 h resulted in restoration of growth, rapid increase in ψwa as well as in the rates of photosynthetic gas exchange, and a sharp decline in the content of soluble sugars and proline. Data on close correspondence between the changes in osmolyte content and ψwa under drought and recovery support the assumption that osmolytes might participate in regulation of ψwa.

Additional key words: drought tolerance; drought stress; malondialdehyde; photosynthesis; pigments.

Received: July 17, 2018; Accepted: June 24, 2019; Prepublished online: July 12, 2019; Published: July 23, 2019  Show citation

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VORONIN, P.Y., MAEVSKAYA, S.N., & NIKOLAEVA, M.K. (2019). Physiological and molecular responses of maize (Zea mays L.) plants to drought and rehydration. Photosynthetica, 57(3), 850-856. doi: 10.32615/ps.2019.101
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    References

    1. Acevedo E., Hsiao T.C., Henderson D.W.: Immediate and subsequent growth response of maize leaves to changes in water status. - Plant Physiol. 48: 631-636, 1971. Go to original source...
    2. Avramova V., AbdElgawad H., Zhang Z. et al.: Drought induces distinct growth response, protection, and recovery mecha-nisms in the maize leaf growth zone. - Plant Physiol. 169: 1382-1396, 2015. Go to original source...
    3. Bates L.S., Waldren R., Teare I.D.: Rapid determination of free proline for water stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    4. Bene¹ová M., Holá D., Fisher L. et al.: The physiology and proteomics of drought tolerance in maize: early stomatal closure as a cause of lower tolerance to short-term dehydra-tion? - PLoS ONE 7: e38017, 2012. Go to original source...
    5. Chaves M.M., Flexas J., Pinheiro C.: Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. - Ann. Bot.-London 103: 551-560, 2009. Go to original source...
    6. Chen D., Wang S., Cao B. et al.: Genotypic variation in growth and physiological response to drought stress and re-watering reveals the critical role of recovery in drought adaptation in maize seedlings. - Front. Plant Sci. 6: 1241, 2016. Go to original source...
    7. Couée I., Sulmon C., Gouesbet G., El Amrani A.: Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. - J. Exp. Bot. 57: 449-459, 2006. Go to original source...
    8. Cruz de Carvalho R., Cunha A., Marques da Silva J.: Photo-synthesis by six Portuguese maize cultivars during drought stress and recovery. - Acta Physiol. Plant. 33: 359-374, 2011.
    9. Flexas J., Bota J., Galmés J. et al.: Keeping a positive carbon balance under adverse conditions: responses of photosyn-thesis and respiration to water stress: A review. - Physiol. Plantarum 127: 343-352, 2006. Go to original source...
    10. Foster K., Lambers H., Real D. et al.: Drought resistance and recovery in mature Bituminaria bituminosa var. albomargi-nata. - Ann. Appl. Biol. 166: 154-169, 2015. Go to original source...
    11. Foyer C.H., Valadier M.H., Migge A., Becker T.W.: Drought-induced effects on nitrate reductase activity and RNA and on the coordination of nitrogen and carbon metabolism in maize leaves. - Plant Physiol. 117: 283-292, 1998. Go to original source...
    12. Ghannoum O.: C4 photosynthesis and water stress. - Ann. Bot.-London 103: 635-644, 2009. Go to original source...
    13. Hare P.D., Cress W.A., Van Staden J.: Dissecting the role of osmolyte accumulation during stress. - Plant Cell Environ. 21: 535-553, 1998. Go to original source...
    14. Hayano-Kanashiro C., Calderón-Vázquez C., Ibarra-Laclett E. et al.: Analysis of gene expression and physiological responses in three Mexican maize landraces under drought stress and recovery irrigation. - PLoS ONE 4: e7531, 2009. Go to original source...
    15. Heath R.L., Packer L.: Photoperoxidation in isolated chloroplasts. -Arch. Biochem. Biophys. 125: 180-198, 1968. Go to original source...
    16. Hsiao T.C.: Plant responses to water stress. - Ann. Rev. Plant Physio. 24: 519-570, 1973. Go to original source...
    17. Kaur G., Asthir B.: Proline: a key player in plant abiotic stress tolerance. - Biol. Plantarum 59: 609-619, 2015. Go to original source...
    18. Kim J.-Y., Mahé A., Brangeon J., Prioul J.L.: A maize vacuolar invertase, IVR 2, is induced by water stress. Organ/tissue specificity and diurnal modulation of expression. - Plant Physiol. 124: 71-84, 2000. Go to original source...
    19. Kosová K., Vítámvás P., Urban M.O. et al.: Plant abiotic stress proteomics: The major factors determining alterations in cellular proteome. - Front. Plant Sci. 9: 122, 2018. Go to original source...
    20. Kuznetsov Vl.V., Shevyakova N.I.: Proline under stress: biological role, metabolism, and regulation. - Russ. J. Plant Physl+ 46: 274-288, 1999.
    21. Lawlor D., Cornic G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficit in higher plants. - Plant Cell Environ. 25: 275-294, 2002. Go to original source...
    22. Lichtenthaler H.K.: Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. - Method. Enzymol. 148: 350-382, 1987. Go to original source...
    23. Maksimov N.A.: [Inhibition of growth processes as the main cause of decreasing yields under drought.] - Adv. Contemp. Biol. 11: 124-136, 1939. [In Russian]
    24. Mittler R., Zilinskas B.A.: Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progres-sion of drought stress and following recovery from drought. -Plant J. 5: 397-405, 1994. Go to original source...
    25. Morgan J.M.: Osmoregulation and water stress in higher plants. -Ann. Rev. Plant Physio. 35: 299-319, 1984. Go to original source...
    26. Muhammadkhani N., Heidari R.: Drought-induced accumulation of soluble sugars and proline in two maize varieties. - World Appl. Sci. J. 3: 448-453, 2008.
    27. Nikolaeva M.K., Maevskaya S.N., Voronin P.Yu.: Photosynthetic CO2/H2O gas exchange and dynamics of carbohydrates con-tent in maize leaves under drought. - Russ. J. Plant Physl+ 64: 536-542, 2017. Go to original source...
    28. Pelleschi S., Rocher J.-P., Prioul J.-L.: Effect of water restriction on carbohydrate metabolism and photosynthesis in mature maize leaves. - J. Cell Environ. 20: 493-503, 1997. Go to original source...
    29. Pisarenko N.F.: [Method for determination of starch and poly-saccharides in cell wall of plants.] - In: Pavlinova O.A. (ed.): [Biochemical Methods in Plant Physiology.] Pp. 35-47. Nauka, Moscow 1971. [In Russian]
    30. Pustovoitova T.N., Zholkevich V.N.: [Main trends in the study of drought effect on physiological processes in plants.] - Fiziol. Biokhim. Kult+ 24: 14-27, 1992. [In Russian]
    31. Reddy A.R., Chaitanya K.V., Vivekanandan M.: Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. - J. Plant Physiol. 161: 1189-1202, 2004. Go to original source...
    32. Sgherri C.L.M., Maffei M., Navari-Izzo F.: Antioxidative enzymes in wheat subjected to increasing water deficit and rewatering. - J. Plant Physiol. 157: 273-279, 2000. Go to original source...
    33. Sharma S., Villamor J.G., Verslues P.E.: Essential role of tissue-specific proline synthesis and catabolism in growth and redox balance at low water potential. - Plant Physiol. 157: 292-304, 2011. Go to original source...
    34. Sicher R.C., Barnaby J.Y.: Impact of carbon dioxide enrichment on the responses maize leaf transcripts and metabolites to water stress. - Physiol. Plantarum 144: 238-253, 2012. Go to original source...
    35. Srivalli B., Sharma G., Khanna-Chopra R.: Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress following recovery. - Physiol. Plantarum 119: 503-512, 2003. Go to original source...
    36. Sun C., Gao X., Chen X. et al.: Metabolic and growth responses of maize to successive drought and re-watering cycles. - Agr. Water Manage. 172: 62-73, 2016. Go to original source...
    37. Tarchevsky I.A.: [Photosynthesis.] - In: Grechkin A.N. (ed.): [Metabolism of Plants under Stress. Selected papers.] Pp. 9-102. Fen, Kazan 2001. [In Russian]
    38. Turkina M.B., Sokolova S.V.: [Methods for monosaccharide and oligosaccharide determination.] - In: Pavlinova O.A. (ed.): [Biochemical Methods in Plant Physiology.] Pp. 7-34. Nauka, Moscow 1971. [In Russian]
    39. Voronin P.Yu.: Experimental installation for measurements of chlorophyll fluorescence, CO2 exchange, and transpiration in a detached leaf. - Russ. J. Plant Physl+ 61: 269-273, 2014. Go to original source...
    40. Voronin P.Yu., Rakhmankulova Z.F., Shuyskaya E.V. et al.: New method for quantitative determination of water potential of mesophyll cell' apoplast in substomatal cavity of the leaf. - Russ. J. Plant Physl+ 64: 452-456, 2017. Go to original source...
    41. Voronin P.Yu., Rakhmankulova Z.F., Tarnopolskaya E.E., Kuznetsov Vl.V.: Closure of stomata in water-stressed pine needles results from the decreased water potential of the mesophyll apoplast in the substomatal cavity. - Russ. J. Plant Physl+ 65: 518-523, 2018. Go to original source...
    42. Zhang J.-Y., Cruz de Carvalho M.H., Torres-Jerez I. et al.: Global reprogramming of transcription and metabolism in Medicago truncatula during progressive drought and after rewatering. - Plant Cell Environ. 37: 2553-2576, 2014. Go to original source...

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