Photosynthetica 2020, 58(SI):262-274 | DOI: 10.32615/ps.2019.148

Special issue in honour of Prof. Reto J. Strasser – A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass)

B. JIMÉNEZ-FRANCISCO1,†, A. STIRBET2,†, G.A. AGUADO-SANTACRUZ3, H. CAMPOS1, F.V. CONDE-MARTÍNEZ1, D. PADILLA-CHACÓN1, C. TREJO1, C.J. BERNACCHI5, G. GOVINDJEE6,a
1 Department of Botany, Postgraduate College, Carretera México-Texcoco km 36.5, Montecillo, 56230 México, México
2 Anne Burras Lane, Newport News, Virginia 23606, USA
3 National Technological Institute of Mexico/IT Roque, Carretera Celaya-Juventino Rosas km. 8, 38110 Celaya, Guanajuato, México
5 USDA-ARS and the Department of Plant Biology, 196 E.R. Madigan Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
6 Department of Biochemistry, Department of Plant Biology, and Center of Biophysics & Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose concentrations, while the photoautotrophic cells showed an optimal PSII activity, close to that of the leaves. Further, our data on cells grown in 0, 0.15, and 0.3% sucrose, but with added CO2, and measured while the cells were deposited on filter paper, indicate that supplementary CO2 can increase the PSII activity in the presence of sucrose, although further research is necessary to understand these results.

Additional key words: kinetic parameters of the O-J, J-I, and I-P phases; osmotic stress; performance index.

Received: July 17, 2019; Accepted: November 5, 2019; Prepublished online: December 12, 2019; Published: May 28, 2020  Show citation

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JIMÉNEZ-FRANCISCO, B., STIRBET, A., CAMPOS, H., CONDE-MARTÍNEZ, F.V., PADILLA-CHACÓN, D., TREJO, C., BERNACCHI, C.J., & GOVINDJEE, G. (2020). Special issue in honour of Prof. Reto J. Strasser – A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass). Photosynthetica, 58(SPECIAL ISSUE), 262-274. doi: 10.32615/ps.2019.148
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