Photosynthetica 2020, 58(3):755-761 | DOI: 10.32615/ps.2020.019

Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy

Y.G. QU1,2, X.L. WANG3, T.Y. KUANG2
1 College of Life Sciences, Linyi University, 276000 Linyi, China
2 Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China
3 College of Life Sciences, Shandong Agricultural University, 271018 Taian, China

Here, the light-induced changes of the core antenna complex chlorophyll protein 43 (CP43) isolated from PSII of spinach were assessed using infrared and terahertz (THz) spectroscopy. We found that the collective vibrational modes at low frequencies and the secondary structures of CP43 were altered upon illumination. The helix changed more than any other secondary structure. CP43 is more easily damaged by illumination than PSII. Infrared and THz spectroscopy have different functions in protein studies. THz spectroscopy has potential in evaluating the stability of proteins.

Additional key words: amide I band; conformation; degradation; photoinhibition.

Received: December 15, 2019; Revised: February 15, 2020; Accepted: February 27, 2020; Prepublished online: April 29, 2020; Published: June 11, 2020  Show citation

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QU, Y.G., WANG, X.L., & KUANG, T.Y. (2020). Light stability of the isolated CP43 studied by infrared and terahertz spectroscopy. Photosynthetica, 58(3), 755-761. doi: 10.32615/ps.2020.019
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