Photosynthetica 2020, 58(5):1188-1199 | DOI: 10.32615/ps.2020.069

Silencing of the receptor-like cytoplasmic kinase gene TaRKL1 reduces photosynthetic capacity in wheat

Y. YING1,2,†, F.F. LIU3,†, G.P. LI3, Q. ZHENG2, B. LI2, Z.S. LI2, J.F. CHENG1, H.W. LI2
1 College of Agronomy, Jiangxi Agricultural University, 330045 Nanchang, Jiangxi, China
2 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Innovative Academy of Seed Design, Chinese Academy of Sciences, 100101 Beijing, China College of Life Sciences, Huaibei Normal University, 235000 Huaibei, Anhui, China3

To explore the role of receptor-like kinases in the regulation of photosynthesis, an uncharacterized TaRKL1 encoding a receptor-like cytoplasmic kinase was investigated in Triticum aestivum cv. Xiaoyan 101 with Barley stripe mosaic virus-induced gene silencing system (BSMV-VIGS). The results showed that the CO2 assimilation rate, stomatal conductance, and transpiration rate were significantly lower in the BSMV:TaRKL1 plants than those in the BSMV:γ00 plants (control). Moreover, the maximum photochemical efficiency and electron transport flux decreased while the dissipated energy flux was enhanced in the BSMV:TaRKL1 plants compared to the control. Additionally, the contents of chlorophylls and carotenoids were reduced in the BSMV:TaRKL1 plants. However, the hydrogen peroxide content was significantly enhanced in the BSMV:TaRKL1 plants, which resulted from lower ascorbate peroxidase activity. Consistent with the inhibition of photosynthesis, the transcription levels of the photosynthesis-related, antioxidant enzymes, senescence-associated genes, and four abscisic acid biosynthesis genes were downregulated substantially. Collectively, this study for the first time showed that TaRKL1 regulates photosynthesis and H2O2 homeostasis. It may be a potential target gene for radiation-use efficiency improvement in wheat.

Additional key words: H2O2; photosynthesis; receptor-like cytoplasmic kinases; stomatal conductance; Triticum aestivum L.; virus-induced gene silencing system.

Received: May 10, 2020; Revised: September 8, 2020; Accepted: September 23, 2020; Prepublished online: October 26, 2020; Published: December 8, 2020  Show citation

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YING, Y., LIU, F.F., LI, G.P., ZHENG, Q., LI, B., LI, Z.S., CHENG, J.F., & LI, H.W. (2020). Silencing of the receptor-like cytoplasmic kinase gene TaRKL1 reduces photosynthetic capacity in wheat. Photosynthetica, 58(5), 1188-1199. doi: 10.32615/ps.2020.069
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