Photosynthetica 2021, 59(1):12-22 | DOI: 10.32615/ps.2020.082

Light quality-dependent regulation of photoprotection and antioxidant properties in rice seedlings grown under different light-emitting diodes

L.H. TRAN†, D.G. LEE†, S. JUNG
School of Life Sciences and Biotechnology, BK21 Plus KNU Creative Bioresearch Group, Kyungpook National University, 41566 Daegu, Korea

We examined light quality-dependent regulation of photoprotection and antioxidant properties in rice seedlings. Seedlings grown under red or green light-emitting diodes (LEDs) showed declines in quantum yield of electron transport through PSII, maximum relative electron transport rates, and photochemical quenching compared to the white and blue LED, but no difference in maximum photochemical efficiency of PSII was found. Blue LED treatment resulted in greater increases in not only nonphotochemical quenching, but also zeaxanthin formation, expressions of β-carotene hydroxylase and violaxanthin de-epoxidase, and anthocyanin compared to other LED treatments. By contrast, green and red LEDs increased activities of superoxide dismutase and ascorbate peroxidase as well as expression levels of catalase (CAT)a and CATb to white and blue LEDs. Green and red LEDs also greatly upregulated expressions of phytochrome (PHY) A, PHYB, PHYC, and cryptochrome 2 compared to white and blue LEDs, whereas blue LED upregulated CRY1. Our results demonstrate that specific wavelengths of different LEDs differentially influence photosynthetic performance, photoprotection, and antioxidant properties, possibly through regulating expressions of phytochrome and cryptochrome genes in a light-quality-dependent manner.

Additional key words: antioxidant enzyme; chlorophyll fluorescence quenching; xanthophyll.

Received: October 2, 2020; Revised: November 23, 2020; Accepted: November 27, 2020; Prepublished online: December 15, 2020; Published: March 18, 2021  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
TRAN, L.H., LEE, D.G., & JUNG, S. (2021). Light quality-dependent regulation of photoprotection and antioxidant properties in rice seedlings grown under different light-emitting diodes. Photosynthetica, 59(1), 12-22. doi: 10.32615/ps.2020.082
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileTran_2644_supplement.docx

    File size: 15.07 kB

    References

    1. Ahmad M., Jarillo J.A., Cashmore A.R.: Chimeric proteins between cry1 and cry2 Arabidopsis blue light photoreceptors indicate overlapping functions and varying protein stability. - Plant Cell 10: 197-207, 1998. Go to original source...
    2. Banerjee R., Schleicher E., Meier S. et al.: The signaling state of Arabidopsis cryptochrome 2 contains flavin semiquinone. - J. Biol. Chem. 282: 14916-14922, 2007. Go to original source...
    3. Baum J.A., Scandalios J.G.: Isolation and characterization of the cytosolic and mitochondrial superoxide dismutases of maize. - Arch. Biochem. Biophys. 206: 249-264, 1981. Go to original source...
    4. Beel B., Prager K., Spexard M. et al.: A flavin binding cryptochrome photoreceptor responds to both blue and red light in Chlamydomonas reinhardtii. - Plant Cell 24: 2992-3008, 2012. Go to original source...
    5. Beers R.F., Sizer I.W.: A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. - J. Biol. Chem. 195: 133-140, 1952. Go to original source...
    6. Bilger W., Björkman O.: Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. - Photosynth. Res. 25: 173-185, 1990. Go to original source...
    7. Bouly J.-P., Schleicher E., Dionisio-Sese M. et al.: Cryptochrome blue light photoreceptors are activated through interconversion of flavin redox states. - J. Biol. Chem. 282: 9383-9391, 2007. Go to original source...
    8. Chalker-Scott L.: Environmental significance of anthocyanins in plant stress responses. - Photochem. Photobiol. 70: 1-9, 1999. Go to original source...
    9. Chatterjee M., Sharma P., Khurana J.P.: Cryptochrome 1 from Brassica napus is up-regulated by blue light and controls hypocotyl/stem growth and anthocyanin accumulation. - Plant Physiol. 141: 61-74, 2006. Go to original source...
    10. Chen G.-X., Asada K.: Ascorbate peroxidase in tea leaves: Occurrence of two isozymes and the differences in their enzymatic and molecular properties. - Plant Cell Physiol. 30: 987-998, 1989.
    11. Colbert J.T., Hershey H.P., Quail P.H.: Phytochrome regulation of phytochrome mRNA abundance. - Plant Mol. Biol. 5: 91-101, 1985. Go to original source...
    12. Consentino L., Lambert S., Martino C. et al.: Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism. - New Phytol. 206: 1450-1462, 2015. Go to original source...
    13. D'Amico-Damião V., Carvalho R.F.: Cryptochrome-related abiotic stress responses in plants. - Front. Plant Sci. 9: 1897, 2018. Go to original source...
    14. Demmig-Adams B., Cohu C.M., Adams III W.W.: Dealing with the hazards of harnessing sunlight. - Nature Education 4: 18, 2012.
    15. Egley G.H., Paul R.N., Vaughn K.C., Duke S.O.: Role of peroxidase in the development of water-impermeable seed coats in Sida spinosa L. - Planta 157: 224-232, 1983. Go to original source...
    16. El-Esawi M., Arthaut L.-D., Jourdan N. et al.: Blue-light induced biosynthesis of ROS contributes to the signaling mechanism of Arabidopsis cryptochrome. - Sci. Rep.-UK 7: 13875, 2017. Go to original source...
    17. Folta K.M., Maruhnich S.A.: Green light: a signal to slow down or stop. - J. Exp. Bot. 58: 3099-3111, 2007. Go to original source...
    18. Foyer C.H., Noctor G.: Redox signaling in plants. - Antioxid. Redox Sign. 18: 2087-2090, 2013. Go to original source...
    19. Foyer C.H., Ruban A.V., Noctor G.: Viewing oxidative stress through the lens of oxidative signalling rather than damage. -Biochem. J. 474: 877-883, 2017. Go to original source...
    20. Genty B., Briantais J.-M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - BBA-Gen. Subjects 990: 87-92, 1989. Go to original source...
    21. Gilmore A.M., Yamamoto H.Y.: Zeaxanthin formation and energy-dependent fluorescence quenching in pea chloro-plasts under artificially mediated linear and cyclic electron transport. - Plant Physiol. 96: 635-643, 1991. Go to original source...
    22. Hakala M., Tuominen I., Keränen M. et al.: Evidence for the role of the oxygen-evolving manganese complex in photoinhibition of Photosystem II. - BBA-Bioenergetics 1706: 68-80, 2005. Go to original source...
    23. Harrison W.G., Platt T.: Photosynthesis-irradiance relationships in polar and temperate phytoplankton populations. - Polar Biol. 5: 153-164, 1986. Go to original source...
    24. He J., Yang W., Qin L. et al.: Photoinactivation of photosystem II in wild-type and chlorophyll b-less barley leaves: which mechanism dominates depends on experimental circumstances. - Photosynth. Res. 126: 399-407, 2015. Go to original source...
    25. Jahns P., Holzwarth A.R.: The role of the xanthophyll cycle and of lutein in photoprotection of photosystem II. - BBA-Bioenergetics 1817: 182-193, 2012. Go to original source...
    26. Jassby A.D., Platt T.: Mathematical formulation of the relationship between photosynthesis and light for phytoplankton. - Limnol. Oceanogr. 21: 540-547, 1976. Go to original source...
    27. Jung S., Kim J.S., Cho K.Y. et al.: Antioxidant responses of cucumber (Cucumis sativus) to photoinhibition and oxidative stress induced by norflurazon under high and low PPFDs. - Plant Sci. 153: 145-154, 2000. Go to original source...
    28. Kondo S., Tomiyama H., Rodyoung A. et al.: Abscisic acid metabolism and anthocyanin synthesis in grape skin are affected by light emitting diode (LED) irradiation at night. - J. Plant Physiol. 171: 823-829, 2014. Go to original source...
    29. Kouøil R., Wientjes E., Bultema J.B. et al.: High-light vs. low-light: Effect of light acclimation on photosystem II composition and organization in Arabidopsis thaliana. - BBA-Bioenergetics 1827: 411-419, 2013. Go to original source...
    30. Kreslavski V.D., Strokina V.V., Pashkovskiy P.P. et al.: Deficiencies in phytochromes A and B and cryptochrome 1 affect the resistance of the photosynthetic apparatus to high-intensity light in Solanum lycopersicum. - J. Photoch. Photobio. B 210: 111976, 2020. Go to original source...
    31. Li X., Wang Q., Yu X. et al.: Arabidopsis cryptochrome 2 (CRY2) functions by the photoactivation mechanism distinct from the tryptophan (trp) triad-dependent photoreduction. - P. Natl. Acad. Sci. USA 108: 20844-20849, 2011. Go to original source...
    32. Ma D., Li X., Guo Y. et al.: Cryptochrome 1 interacts with PIF4 to regulate high temperature-mediated hypocotyl elongation in response to blue light. - P. Natl. Acad. Sci. USA 113: 224-229, 2016. Go to original source...
    33. Mancinelli A.L., Yang C.-P.H., Lindquist P. et al.: Photocontrol of anthocyanin synthesis. III. The action of streptomycin on the synthesis of chlorophyll and anthocyanin. - Plant Physiol. 55: 251-257, 1975. Go to original source...
    34. Mitchell C., Both A.J., Bourget C. et al.: LEDs: The future of greenhouse lighting. - Chron. Horticult. 52: 6-12, 2012.
    35. Mohr H.: Coaction between pigment systems. - In: Kendrick R.E., Kronenberg G.H.M. (ed.): Photomorphogenesis in Plants. 2nd Edition. Pp. 353-373. Springer, Dordrecht 1994. Go to original source...
    36. Murchie E.H., Lawson T.: Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. - J. Exp. Bot. 64: 3983-3998, 2013. Go to original source...
    37. Nakabayashi R., Yonekura-Sakakibara K., Urano K. et al.: Enhancement of oxidative and drought tolerance in Arabi-dopsis by overaccumulation of antioxidant flavonoids. -Plant J. 77: 367-379, 2014. Go to original source...
    38. Neff M.M., Chory J.: Genetic interactions between phytochrome A, phytochrome B, and cryptochrome 1 during Arabidopsis development. - Plant Physiol. 118: 27-35, 1998. Go to original source...
    39. Noctor G., Mhamdi A., Foyer C.H.: The roles of reactive oxygen metabolism in drought: Not so cut and dried. - Plant Physiol. 164: 1636-1648, 2014. Go to original source...
    40. Olson P.D., Varner J.E.: Hydrogen peroxide and lignification. - Plant J. 4: 887-892, 1993. Go to original source...
    41. Rao M.V., Paliyath G., Ormrod D.P.: Ultraviolet-B- and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana. - Plant Physiol. 110: 125-136, 1996. Go to original source...
    42. Ruban A.V.: Nonphotochemical chlorophyll fluorescence quenching: Mechanism and effectiveness in protecting plants from photodamage. - Plant Physiol. 170: 1903-1916, 2016. Go to original source...
    43. Rusaczonek A., Czarnocka W., Kacprzak S. et al.: Role of phytochromes A and B in the regulation of cell death and acclimatory responses to UV stress in Arabidopsis thaliana. -J. Exp. Bot. 66: 6679-6695, 2015. Go to original source...
    44. Schreiber U., Schliwa U., Bilger W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. - Photosynth. Res. 10: 51-62, 1986. Go to original source...
    45. Sharma R., Sopory S.K., Guha-Mukherjee S.: Phytochrome regulation of peroxidase activity in maize. - Plant Sci. Lett. 6: 69-75, 1976. Go to original source...
    46. Suzuki N., Koussevitzky S., Mittler R., Miller G.: ROS and redox signalling in the response of plants to abiotic stress. - Plant Cell Environ. 35: 259-270, 2012. Go to original source...
    47. Tran L.H., Jung S.: Effects of light-emitting diode irradiation on growth characteristics and regulation of porphyrin biosynthesis in rice seedlings. - Int. J. Mol. Sci. 18: 641, 2017. Go to original source...
    48. van Wijk K.J., van Hasselt P.R.: Photoinhibition of photosystem II in vivo is preceded by down-regulation through light-induced acidification of the lumen: Consequences for the mechanism of photoinhibition in vivo. - Planta 189: 359-368, 1993. Go to original source...
    49. Wang F., Wu N., Zhang L. et al.: Light signaling-dependent regulation of photoinhibition and photoprotection in tomato. -Plant Physiol. 176: 1311-1326, 2018. Go to original source...
    50. Wang H., Deng X.W.: Dissecting the phytochrome A-dependent signaling network in higher plants. - Trends Plant Sci. 8: 172-178, 2003. Go to original source...
    51. Woodbury W., Spencer A.K., Stahmann M.A.: An improved procedure using ferricyanide for detecting catalase isozymes. -Anal. Biochem. 44: 301-305, 1971. Go to original source...
    52. Wu H.: Effect of different light qualities on growth, pigment content, chlorophyll fluorescence, and antioxidant enzyme activity in the red alga Pyropia haitanensis (Bangiales, Rhodophyta). - BioMed Res. Int. 2016: 7383918, 2016. Go to original source...
    53. Wu M.-C., Hou C.Y., Jiang C.M. et al.: A novel approach of LED light radiation improves the antioxidant activity of pea seedlings. - Food Chem. 101: 1753-1758, 2007. Go to original source...
    54. Yu W., Liu Y., Song L. et al.: Effect of differential light quality on morphology, photosynthesis, and antioxidant enzyme activity in Camptotheca acuminata seedlings. - J. Plant Growth Regul. 36: 148-160, 2017. Go to original source...
    55. Zavafer A., Cheah M.H., Hillier W. et al.: Photodamage to the oxygen evolving complex of photosystem II by visible light. -Sci. Rep.-UK 5: 16363, 2015. Go to original source...
    56. Zhang T.-J., Chow W.S., Liu X.-T. et al.: A magic red coat on the surface of young leaves: anthocyanins distributed in trichome layer protect Castanopsis fissa leaves from photoinhibition. - Tree Physiol. 36: 1296-1306, 2016. Go to original source...
    57. Zhang Y., Jiang L., Li Y. et al.: Effect of red and blue light on anthocyanin accumulation and differential gene expression in strawberry (Fragaria × ananassa). - Molecules 23: 820, 2018. Go to original source...

    Return to the content