Photosynthetica 2021, 59(2):245-255 | DOI: 10.32615/ps.2021.015

Application of fast light-readapted plants for measurement of chlorophyll fluorescence and P700 light absorption with the RLC method

E.A. LYSENKO
Institute of Plant Physiology RAS, 35 Botanicheskaya St., 127276 Moscow, Russia

The rapid light curve method is a time-saving approach to the measurement of photosynthetic processes over a wide spectrum of light intensities. The adaptation to darkness enables the calculation of a complete set of coefficients for chlorophyll (Chl) fluorescence and P700 light absorption; however, dark-adapted plants demonstrate drastically different patterns of Chl fluorescence than that of light-adapted plants. The present work compared light-adapted barley plants without dark adaptation (L), and barley plants adapted to darkness and readapted to light for 7.5 min (D/L). The rapid light curves were generally similar in both the L and D/L variants. Some differences between the L and D/L variants were found in plants grown at any illumination. A number of distinctions between the L and D/L variants were characteristic of plants grown under particular illumination levels. The present analysis demonstrated that the scheme including dark adaptation and short readaptation to light (D/L) enabled to obtain generally correct data.

Additional key words: actinic light intensity; barley; pulse amplitude modulation; rapid light curves.

Received: November 5, 2020; Revised: February 24, 2021; Accepted: March 3, 2021; Prepublished online: April 12, 2021; Published: June 29, 2021  Show citation

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LYSENKO, E.A. (2021). Application of fast light-readapted plants for measurement of chlorophyll fluorescence and P700 light absorption with the RLC method. Photosynthetica, 59(2), 245-255. doi: 10.32615/ps.2021.015
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