PHOTOSYNTHETICA

Photosynthetica 2023, 61(2):215-224 | DOI: 10.32615/ps.2023.009

Impact of additional green light and deficit in cryptochrome 1 on photosynthetic activity and pro-/antioxidant balance in Arabidopsis thaliana

V. KRESLAVSKI¹, A. KHUDYAKOVA¹, A. KOSOBRYUKHOV¹, P. PASHKOVSKIY², M. VERESHCHAGIN², T. BALAKHNINA¹, H.F. ALHARBY³, S.I. ALLAKHVERDIEV^{1, 2, 4}

¹ Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, 142290 Pushchino, Moscow Region, Russia

² K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia

³ Faculty of Science, King Abdulaziz University, 21589 Jeddah, Saudi Arabia

⁴ Faculty of Engineering and Natural Sciences, Bahcesehir University, Istanbul, Turkey

The light spectral composition acting through a set of photoreceptors, such as cryptochromes and phytochromes, plays an important role in maintaining sustainable photosynthesis. An impact of cryptochrome 1 deficiency and additions of green light (GL) against the background of red (RL) and blue (BL) (different ratios of RL:BL:GL) on the activity of the photosynthetic apparatus, the content of photosynthetic pigments, pro-/antioxidant balance, and expression of some genes in the leaves of 23-d-old Arabidopsis thaliana hy4 mutant plants was studied. The deficiency of cryptochrome 1 at RL/BL ratio of 4:1 led to a decrease in the rate of photosynthesis, photosystem II activity, and activity of ascorbate peroxidase and total peroxidase but to an increase in the content of products reacting with thiobarbituric acid. However, in the presence of additional GL, this difference for photosynthetic parameters either decreased or was absent, likely due to a GL-induced decrease in the content of active cryptochrome.

Additional key words: cryptochrome mutant; photosynthesis; photosystem II.

Received: February 4, 2023; Revised: March 3, 2023; Accepted: March 14, 2023; Prepublished online: March 24, 2023; Published: June 6, 2023  Show citation