PHOTOSYNTHETICA

Photosynthetica 2024, 62(1):71-78 | DOI: 10.32615/ps.2024.009

Effect of cryptochrome 1 deficiency and spectral composition of light on photosynthetic processes in A. thaliana under high-intensity light exposure

A. KHUDYAKOVA¹, V. KRESLAVSKI¹, A. KOSOBRYUKHOV¹, M. VERESHAGIN², S.I. ALLAKHVERDIEV^{1, 2, 3}

¹ Institute of Basic Biological Problems, Russian Academy of Sciences, Institutskaya Street 2, Pushchino, 142290 Moscow Region, Russia

² K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia

³ Faculty of Engineering and Natural Sciences, Bahçeşehir University, Istanbul, Turkey

The role of cryptochrome 1 in photosynthetic processes and pro-/antioxidant balance in the Arabidopsis thaliana plants was studied. Wild type (WT) and hy4 mutant deficient in cryptochrome 1 grown for 20 d under red (RL, 660 nm) and blue (BL, 460 nm) light at an RL:BL = 4:1 ratio were kept for 3 d in different lights: RL:BL = 4:1, RL:BL:GL = 4:1:0.3 (GL - green light, 550 nm), and BL, then were exposed to high irradiance (4 h). Activity of PSII and the rate of photosynthesis in WT and hy4 decreased under the high irradiance in all spectral variants but under BL stronger decrease in the activity was found in the hy4 mutant than in WT. We assumed that lowered resistance of photosynthetic apparatus in the hy4 mutant may be associated with the low activity of the main antioxidant enzymes and reduced content of low-molecular-mass antioxidants in the mutant compared to the WT.

Additional key words: Arabidopsis thaliana; cryptochrome; high-intensity light; photosynthesis; pro-, antioxidant balance.

Received: October 21, 2023; Revised: December 28, 2023; Accepted: January 24, 2024; Prepublished online: February 5, 2024; Published: February 22, 2024  Show citation