Photosynthetica 2000, 38(4):607-614 | DOI: 10.1023/A:1012469709374

Photosystem 2 Efficiency and Thylakoid Protein Pattern in DCMU-Treated Wheat Seedlings during Senescence

N. Nedunchezhian1, K. Muthuchelian2, M. Bertamini3
1 Instituto Agrario de San Michele all' Adige, San Michele all' Adige, Italy
2 School of Energy, Environment and Natural Resources, Madurai Kamaraj University, Madurai, India
3 Instituto Agrario de San Michele all' Adige, San Michele all' Adige, Italy

Changes in various components of photosynthetic apparatus during the 6-d dark incubation at 25 °C of detached control and DCMU-treated Triticum aestivum L. leaves were examined. The rate of photosystem 2 (PS2) activity was decreased with increase of the time of dark incubation in control leaves. In contrast to this, DCMU-treated leaves demonstrated high stability by slowing down the inactivation processes. Diphenyl carbazide and NH2OH restored the PS2 activity more in control leaves than in DCMU-treated leaves. Mn2+ failed to restore the PS2 activity in both control and DCMU-treated samples. Similar results were obtained when Fv/Fm was evaluated by chlorophyll fluorescence measurements. The marked loss of PS2 activity in dark incubated control leaves was primarily due to the loss of D1, 33, and 23 kDa extrinsic polypeptides and 28-25 kDa LHCP2 polypeptides.

Additional key words: chlorophyll fluorescence; diphenyl carbazide; donor side; electron transport; MnCl2; NH2OH; photosystem; senescence; thylakoid protein; Triticum aestivum

Prepublished online: August 1, 2000; Published: November 1, 2000  Show citation

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Nedunchezhian, N., Muthuchelian, K., & Bertamini, M. (2000). Photosystem 2 Efficiency and Thylakoid Protein Pattern in DCMU-Treated Wheat Seedlings during Senescence. Photosynthetica, 38(4), 607-614. doi: 10.1023/A:1012469709374
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