Photosynthetica 2022, 60(1):88-101 | DOI: 10.32615/ps.2022.003

Variability of the photosynthetic machinery tolerance when imposed to rapidly or slowly imposed dehydration in native Mediterranean plants

E. KOUTRA1, C. CHONDROGIANNIS2, G. GRAMMATIKOPOULOS2
1 Laboratory of Biochemical Engineering & Environmental Technology (LBEET), Department of Chemical Engineering, University of Patras, Patras, Greece
2 Laboratory of Plant Physiology, Department of Biology, University of Patras, 26504 Patras, Greece

Dehydration affects the photosynthetic apparatus. The impact of dehydration on photosynthesis was assessed in twelve Mediterranean species representing different growth forms. Rapid and slow dehydration experiments were conducted to (1) compare the impact of water stress among species and growth forms, (2) rank species according to their drought tolerance. Rapid dehydration reduced the electron transport up to PSI, the reduction being linearly related to leaf relative water content (RWC), except for the deciduous species. Specific energy fluxes per reaction center and maximum photochemical activity of PSII remained relatively stable until 10-30% RWC. The modification pattern of the studied parameters was similar for all the growth forms. Slow rehydration increased specific energy fluxes and decreased quantum yields. The dehydration pattern was similar among growth forms, while the recovery pattern was species-specific. Drought tolerance ranking through drought factor index was relatively modified with the integrated biomarker response method.

Additional key words: drought factor index; integrated biomarker response; JIP-test; photosynthesis; plant growth forms; water stress.

Received: October 4, 2021; Revised: December 30, 2021; Accepted: January 12, 2022; Prepublished online: February 23, 2022; Published: March 18, 2022  Show citation

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KOUTRA, E., CHONDROGIANNIS, C., & GRAMMATIKOPOULOS, G. (2022). Variability of the photosynthetic machinery tolerance when imposed to rapidly or slowly imposed dehydration in native Mediterranean plants. Photosynthetica, 60(SPECIAL ISSUE 2022), 88-101. doi: 10.32615/ps.2022.003
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    References

    1. Bednaříková M., Váczi P., Lazár D., Barták M.: Photosynthetic performance of Antarctic lichen Dermatocarpon polyphyllizum when affected by desiccation and low temperatures. - Photosynth. Res. 145: 159-177, 2020. Go to original source...
    2. Beliaeff B., Burgeot T.: Integrated biomarker response: A useful tool for ecological risk assessment. - Environ. Toxicol. Chem. 21: 1316-1322, 2002. Go to original source...
    3. Broeg K., Lehtonen K.K.: Indices for the assessment of environmental pollution of the Baltic Sea coasts: Integrated assessment of a multi-biomarker approach. - Mar. Pollut. Bull. 53: 508-522, 2006. Go to original source...
    4. Bussotti F.: Assessment of stress conditions in Quercus ilex L. leaves by O-J-I-P chlorophyll a fluorescence analysis. - Plant Biosyst. 138: 101-109, 2004. Go to original source...
    5. Campos H., Trejo C., Peña-Valdivia C.B. et al.: Stomatal and non-stomatal limitations of bell pepper (Capsicum annuum L.) plants under water stress and re-watering: Delayed restoration of photosynthesis during recovery. - Environ. Exp. Bot. 98: 56-64, 2014. Go to original source...
    6. Ceppi M.G., Oukarroum A., Çiçek N. et al.: The IP amplitude of the fluorescence rise OJIP is sensitive to changes in the photosystem I content of leaves: A study on plants exposed to magnesium and sulfate deficiencies, drought stress and salt stress. - Physiol. Plantarum 144: 277-288, 2012. Go to original source...
    7. Chaves M.M., Flexas J., Pinheiro C.: Photosynthesis under drought and salt stress: Regulation mechanisms from whole plant to cell. - Ann. Bot.-London 103: 551-560, 2009. Go to original source...
    8. Chaves M.M., Maroco J.P., Pereira J.S.: Understanding plant responses to drought - from genes to the whole plant. - Funct. Plant Biol. 30: 239-264, 2003. Go to original source...
    9. Chondrogiannis C., Grammatikopoulos G.: Photosynthesis in developing leaf of juveniles and adults of three Mediterranean species with different growth forms. - Photosynth. Res. 130: 427-444, 2016. Go to original source...
    10. Chondrogiannis C., Grammatikopoulos G.: Transition from juvenility to maturity strengthens photosynthesis in sclerophyllous and deciduous but not in semi-deciduous Mediterranean shrubs. - Environ. Exp. Bot. 180: 104265, 2021. Go to original source...
    11. Dekov I., Tsonev T., Yordanov I.: Effects of water stress and high-temperature stress on the structure and activity of photosynthetic apparatus of Zea mays and Helianthus annuus. - Photosynthetica 38: 361-366, 2000. Go to original source...
    12. Duarte B., Pedro S., Marques J.C. et al.: Zostera noltii development probing using chlorophyll a transient analysis (JIP-test) under field conditions: Integrating physiological insights into a photochemical stress index. - Ecol. Indic. 76: 219-229, 2017. Go to original source...
    13. Ferreira N.G.C., Morgado R., Santos M.J.G. et al.: Biomarkers and energy reserves in the isopod Porcellionides pruinosus: The effects of long-term exposure to dimethoate. - Sci. Total Environ. 502: 91-102, 2015. Go to original source...
    14. Flexas J., Barbour M.M., Brendel O. et al.: Mesophyll diffusion conductance to CO2: An unappreciated central player in photosynthesis. - Plant Sci. 193-194: 70-84, 2012. Go to original source...
    15. Flexas J., Bota J., Cifre J. et al.: Understanding down-regulation of photosynthesis under water stress: future prospects and searching for physiological tools for irrigation management. - Ann. Appl. Biol. 144: 273-283, 2004. Go to original source...
    16. Flexas J., Diaz-Espejo A., Gago J. et al.: Photosynthetic limitations in Mediterranean plants: A review. - Environ. Exp. Bot. 103: 12-23, 2014. Go to original source...
    17. Flexas J., Medrano H.: Drought-inhibition of photosynthesis in C3 plants: Stomatal and non-stomatal limitations revisited. - Ann. Bot.-London 89: 183-189, 2002. Go to original source...
    18. Galmés J., Flexas J., Medrano H. et al.: Ecophysiology of photosynthesis in semi-arid environments. - In: Flexas J., Loreto F., Medrano H. (ed.): Terrestrial Photosynthesis in a Changing Environment. A Molecular, Physiological and Ecological Approach. Pp. 448-464. Cambridge University Press, Cambridge 2012. Go to original source...
    19. Galmés J., Medrano H., Flexas J.: Photosynthetic limitations in response to water stress and recovery in Mediterranean plants with different growth forms. - New Phytol. 175: 81-93, 2007. Go to original source...
    20. Goltsev V., Zaharieva I., Chernev P. et al.: Drought-induced modifications of photosynthetic electron transport in intact leaves: analysis and use of neural networks as a tool for a rapid non-invasive estimation. - BBA-Bioenergetics 1817: 1490-1498, 2012. Go to original source...
    21. Gomes M.T.G., da Luz A.C., dos Santos M.R. et al.: Drought tolerance of passion fruit plants assessed by the OJIP chlorophyll a fluorescence transient. - Sci. Hortic.-Amsterdam 142: 49-56, 2012. Go to original source...
    22. Grammatikopoulos G., Kyparissis A., Manetas Y.: Seasonal and diurnal gas exchange characteristics and water relations of the drought semi-deciduous shrub Phlomis fruticosa L. under Mediterranean field conditions. - Flora 190: 71-78, 1995. Go to original source...
    23. Grammatikopoulos G., Manetas Y.: Direct absorption of water by hairy leaves of Phlomis fruticosa and its contribution to drought avoidance. - Can. J. Bot. 72: 1805-1811, 1994. Go to original source...
    24. Guha A., Sengupta D., Reddy A.R.: Polyphasic chlorophyll a fluorescence kinetics and leaf protein analyses to track dynamics of photosynthetic performance in mulberry during progressive drought. - J. Photoch. Photobio. B 119: 71-83, 2013. Go to original source...
    25. Kaiser W.M.: Effects of water deficit on photosynthetic capacity. -Physiol. Plantarum 71: 142-149, 1987. Go to original source...
    26. Kalaji H.M., Jajoo A., Oukarroum A. et al.: Chlorophyll a fluorescence as a tool to monitor physiological status of plants under abiotic stress conditions. - Acta. Physiol. Plant. 38: 102, 2016. Go to original source...
    27. Karavatas S., Manetas Y.: Seasonal patterns of photosystem 2 photochemical efficiency in evergreen sclerophylls and drought semi-deciduous shrubs under Mediterranean field conditions. - Photosynthetica 36: 41-49, 1999. Go to original source...
    28. Koller S., Holland V., Brüggemann W.: Effects of drought stress on the evergreen Quercus ilex L., the deciduous Q. robur L. and their hybrid Q. × turneri Willd. - Photosynthetica 51: 574-582, 2013. Go to original source...
    29. Lauriano J.A., Lidon F.C., Carvalho C.A. et al.: Drought effects on membrane lipids and photosynthetic activity in different peanut cultivars. - Photosynthetica 38: 7-12, 2000. Go to original source...
    30. Lawlor D.W., Cornic G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. - Plant Cell Environ. 25: 275-294, 2002. Go to original source...
    31. Lawlor D.W., Tezara W.: Causes of decreased photosynthetic rate and metabolic capacity in water-deficient leaf cells: a critical evaluation of mechanisms and integration of processes. - Ann. Bot.-London 103: 561-579, 2009. Go to original source...
    32. Lazár D.: The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light. - Funct. Plant Biol. 33: 9-30, 2006. Go to original source...
    33. Lazár D, Nauą J.: Statistical properties of chlorophyll fluorescence induction parameters. - Photosynthetica 35: 121-127, 1998. Go to original source...
    34. Matos M.C., Campos P.S., Ramalho J.C. et al. Photosynthetic activity and cellular integrity of the Andean legume Pachyrhizus ahipa (Wedd.) Parodi under heat and water stress. - Photosynthetica 40: 493-501, 2002. Go to original source...
    35. Nogués S., Alegre L.: An increase in water deficit has no impact on the photosynthetic capacity of field-grown Mediterranean plants. - Funct. Plant Biol. 29: 621-630, 2002. Go to original source...
    36. Oukarroum A., Madidi S.E., Schansker G., Strasser R.J.: Probing the responses of barley cultivars (Hordeum vulgare L.) by chlorophyll a fluorescence OLKJIP under drought stress and re-watering. - Environ. Exp. Bot. 60: 438-446, 2007. Go to original source...
    37. Oukarroum A., Schansker G., Strasser R.J.: Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance. - Physiol. Plantarum 137: 188-199, 2009. Go to original source...
    38. Oukarroum A., Strasser R.J., Schansker G.: Heat stress and the photosynthetic electron transport chain of the lichen Parmelina tiliacea (Hoffm.) Ach. in the dry and the wet state: differences and similarities with the heat stress response of higher plants. - Photosynth. Res. 111: 303-314, 2012. Go to original source...
    39. Petsas A., Grammatikopoulos G.: Drought resistance and recovery of photosystem II activity in a Mediterranean semi-deciduous shrub at the seedling stage. - Photosynthetica 47: 284-292, 2009. Go to original source...
    40. Redillas M.C.F.R., Strasser R.J., Jeong J.S. et al.: The use of JIP test to evaluate drought-tolerance of transgenic rice overexpressing OsNAC10. - Plant Biotechnol. Rep. 5: 169-175, 2011. Go to original source...
    41. Stirbet A., Govindjee: On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and Photosystem II: Basics and applications of the OJIP fluorescence transient. - J. Photoch. Photobio. B. 104: 236-257, 2011. Go to original source...
    42. Stirbet A., Lazár D., Kromdijk J., Govindjee: Chlorophyll a fluorescence induction: Can just a one-second measurement be used to quantify abiotic stress responses? - Photosynthetica 56: 86-104, 2018. Go to original source...
    43. Strasser R.J., Stirbet A.D.: Heterogeneity of photosystem II probed by the numerically simulated chlorophyll a fluorescence rise (O-J-I-P). - Math. Comput. Simulat. 48: 3-9, 1998. Go to original source...
    44. Strasser R.J., Tsimilli-Michael M., Qiang S., Goltsev V.: Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. - BBA-Bioenergetics 1797: 1313-1326, 2010. Go to original source...
    45. Strasser R.J., Tsimilli-Michael M., Srivastava A.: The fluorescence transient as a tool to characterize and screen photosynthetic samples. - In: Yunus M., Pathre U., Mohanty P. (ed.): Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Pp. 443-480. Taylor & Francis, London 2000.
    46. Strasser R.J., Tsimilli-Michael M., Srivastava A.: Analysis of the chlorophyll a fluorescence transient. - In: Papageorgiou G.C., Govindjee (ed.): Chlorophyll a Fluorescence: A Signature of Photosynthesis. Advances in Photosynthesis and Respiration. Pp. 321-362. Springer, Dordrecht 2004. Go to original source...
    47. Tseliou E., Chondrogiannis C., Kalachanis D. et al.: Integration of biophysical photosynthetic parameters into one photochemical index for early detection of Tobacco Mosaic Virus infection in pepper plants. - J. Plant Physiol. 267: 153542, 2021. Go to original source...
    48. Tsimilli-Michael M.: Revisiting JIP-test: An educative review on concepts, assumptions, approximations, definitions and terminology. - Photosynthetica 58: 275-292, 2020. Go to original source...
    49. Tsimilli-Michael M., Strasser R.J.: In vivo assessment of plants' vitality: applications in detecting and evaluating the impact of mycorrhization on host plants. - In: Varma A. (ed.): Mycorrhiza: State of the Art. Genetics and Molecular Biology, Eco-Function, Biotechnology, Eco-Physiology, Structure and Systematics, 3rd Edition. Pp. 679-703. Springer, Berlin-Heidelberg 2008. Go to original source...
    50. Turner N.C.: Techniques and experimental approaches for the measurement of plant water status. - Plant Soil 58: 339-366, 1981. Go to original source...
    51. van Heerden P.D.R., Swanepoel J.W., Krüger G.H.J.: Modulation of photosynthesis by drought in two desert scrub species exhibiting C3-mode CO2 assimilation. - Environ. Exp. Bot. 61: 124-136, 2007. Go to original source...
    52. ®ivčák M., Brestič M., Kalaji H.M., Govindjee: Photosynthetic responses of sun- and shade-grown barley leaves to high light: is the lower PSII connectivity in shade leaves associated with protection against excess of light? - Photosynth. Res. 119: 339-354, 2014. Go to original source...
    53. ®ivčák M., Brestič M., Oląovská K., Slamka P.: Performance index as a sensitive indicator of water stress in Triticum aestivum L. - Plant Soil Environ. 54: 133-139, 2008. Go to original source...

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