Photosynthetica 2024, 62(4):361-371 | DOI: 10.32615/ps.2024.039

Ascorbic acid is involved in melatonin-induced salinity tolerance of maize (Zea mays L.) by regulating antioxidant and photosynthetic capacities

M. ZHU1, †, T. GUO1, †, Y.B. LIU1, R. XIAO1, T. YU1, J.X. HUANG1, W.L. DU1, X.M. ZHONG1, B. SONG2, F.H. LI1
1 College of Agronomy, Specialty Corn Institute, Shenyang Agricultural University, 110866 Shenyang, Liaoning Province, China
2 Affiliated Experimental Field, Shenyang Agricultural University, 110866 Shenyang, Liaoning Province, China

Melatonin (MT), an indole compound, can boost plant growth under abiotic stress conditions. This experiment aims to elucidate the synergistic effect of MT and ascorbic acid (AsA) in mitigating salinity stress by assessing the photosynthetic and antioxidant capacity of the maize inbred lines H123 and W961. The results indicated that exogenous MT and AsA significantly improved photosynthetic efficiency and biomass of maize under salinity stress. Additionally, exogenous MT and AsA also improved antioxidant enzyme activities, promoted regeneration of AsA and GSH, decreased reactive oxygen species contents, suppressed Na+ accumulation, and improved the K+/Na+ ratio of maize seedlings. Additionally, the AsA inhibitor lycorine decreased the endogenous content of AsA and eliminated the positive effects of MT, while the MT inhibitor p-chlorophenyl alanine (CPA) reduced the endogenous content of MT, which could not eliminate the promoting effects of AsA. The results suggested that AsA may act as a downstream signal involved in the regulatory effects of MT on maize under salinity stress.

Additional key words: AsA-GSH cycle; ascorbic acid; ionic homeostasis; maize; melatonin; salinity stress.

Received: July 15, 2024; Revised: November 8, 2024; Accepted: November 12, 2024; Prepublished online: December 3, 2024; Published: December 19, 2024  Show citation

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ZHU, M., GUO, T., LIU, Y.B., XIAO, R., YU, T., HUANG, J.X., ... LI, F.H. (2024). Ascorbic acid is involved in melatonin-induced salinity tolerance of maize (Zea mays L.) by regulating antioxidant and photosynthetic capacities. Photosynthetica, 62(4), 361-371. doi: 10.32615/ps.2024.039
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